Objective:To investigate the roles of lipoxin A4 (LXA4) in attenuating myocardial hypoxia/reoxygenation(H/R) lesion and the possible mechanisms. Methods:The cells were randomly divided into five groups:control group,H/R group,LXA4 + H/R group,ZnPP + H/R group,LXA4 + ZnPP + H/R group. Pathological changes of cells was observed. The levels of LDH and CK in cellular supernatants were measured,and activity of HO-1 was measured. HO-1 mRNA expression was analyzed by RT-PCR. Results:Pretreatment of the cells undergoing hypoxia/reoxygenation lesion with LXA4 significantly reduced the LDH and CK levels of cells,protected cells from necrosis,and increased the HO-1 activity and the HO-1 mRNA expression as compared with those in the cells without LXA4 pretreatment. However,HO-1 inhibition by ZnPP abolished the protective role of LXA4 on the cells undergoing hypoxia/reoxygenation lesion. Conclusion:LXA4 can induce HO-1 overexpression which provide the protective role on myocardiac cells undergoing hypoxia/reoxygenation lesion.