Objective: To understand HIV infection of men who have sex with men(MSM) in Jangsu province,and to estimate the annual HIV incidence of MSM. Methods:Total 3 152 samples from 3 rounds survey in 3 cities(Nanjing,Yangzhou and Suzhou)among MSM during 2008-2009 were tested to find HIV-1 seropositive samples. HIV-1 seropositive samples were tested with BED HIV-1 IgG capture enzyme immunoassay(BED-CEIA) to find recent HIV-1 infections and to estimate the annual HIV-1 incidence of MSM. Results: There were 175 samples found to be HIV-1 seropositive,and 92 samples confirmed to be recently infected.The annual HIV-1 incidence of 3 rounds survey were 7.05%,5.18% and 3.71% in Nanjing,6.99%,2.39% and 12.27% in Yangzhou,6.73%,3.31% and 9.84% in Suzhou. Conclusion: The prevalence of HIV-1 in MSM in Jiangsu province was still at a high level. Powerful prevention and control effort must be made.