Objective:To explore economic and efficient way to block perinatal transmission of HBV by passive-active immunoprophylaxis with different doses of hepatitis B immunoglobulin (HBIG) in newborns with HBsAg positive mothers in China. Methods:The newborns with HBsAg-positive mothers were randomly divided into 2 groups and received 100 IU and 200 IU HBIG respectively within 12 hours after birth. Meanwhile all the newborns were received 3 doses of 10 μg yeast recombinant hepatitis B vaccine (HB vaccine) according to EPI procedure. Post-vaccination tests to detect HBsAg and anti-HBs were assessed at 7 months of age. Results:The success rate of blocking perinatal transmission in newborns of 100 IU and 200 IU group were 98.3% and 98.0% respectively. The difference of blocking efficacy between the two groups was not significant,and the HBsAg positive rates between the two groups were also not significantly different when combined with analysis of mothers’ HBeAg status. Conclusion:Combined with 10 μg yeast recombinant hepatitis B vaccine,the passive-active immunoprophylaxis using 100 IU HBIG was similar to 200 IU HBIG to prevent HBV infection of infant with HBsAg positive mother.