Objective:To observe the functional changes of murine bone marrow derived dendritic cells (DCs) loaded with the soluble egg antigen (SEA),and its impact on the differentiation of Th17 cell. Methods:Murine bone marrow cells were induced to differentiate into DCs by GM-CSF and IL-4. Flow cytometry was used to measure IL-6 and IL-23 and ELISA was used to measure TGF-β concentrations in the supernatant of cultured DCs. CD4+T cells and DCs were co-cultured and then the concentrations of relevant cytokines in the culture supernatant were detected. Results:Highly purified DCs were induced and could be used for further experiments. DCs were stimulated with SEA to express high levels of CD40,CD80,CD86 and MHC-II. Meanwhile,DCs stimulated with SEA produced significantly higher concentrations of IL-6 and TGF-β than the blank control group. Co-cultured cells stimulated with SEA yielded significantly higher concentrations of IL-17 than the blank control group. Conclusion:Stimulation by SEA generates mature DCs,and can induce the differentiation of CD4+T cells towards Th17 cells.