Objective:To observe the expression of lactate dehydrogenase-A(LDH-A) in cholangiocarcinoma tissues,human cholangiocarcinoma cell line HuCCT1 and human normal bile duct epithelium cell line HIBEpic. Methods: The expression of LDH-A in 32 cholangiocarcinoma and 15 adjacent non-cancerous tissues was analyzed by immunohistochemistry. RT-PCR and Western blotting assays were used to detect the expression of LDH-A in human cholangiocarcinoma cell line HuCCT1 and human normal bile duct epithelium cell line HIBEpic. Results: LDH-A was overexpressed in 56.3% (18/32) cholangiocarcinoma tissues (P < 0.01) and in HuCCT1 cells but not in HIBEpic cells. Conclusion: Cholangiocarcinoma tissues and human cholangiocarcinoma cell lines HuCCT1 overexpress LDH-A,indicating that LDH-A may play an important role in the initiation and progression of cholangiocarcinoma.