Objective:To explore the effect of fluvastatin(Flu) on the expression of fibronectin(FN) in human peritoneal mesothelial cells (HPMCs) induced by high-glucose peritoneal dialysate (HGPDS). Methods:After incubated in DMEM with 0.01%FBS for 24 h,cultured HPMCs were randomly divided into control group,HGPDS group,HGPDS+Flu group,Flu alone group and DMSO control group. HPMCs were treated for 3,6,12,24,36,48,72 h respectively and the cellular viability were detected by MTT colorimetry. The mRNA expression of FN was observed by RT-PCR. HPMCs were treated for 24 h and the levels of FN in the culture media were measured by ELISA. Results:The results of MTT revealed that lower viability was found in the cells treated with HGPDS and partialy restored in cells treated with different concentrations of Flu at 24 h and 36 h when compared with control group. The significant improvement of cell viability was shown in Flu treatment at concentration of 1 × 10-6 mol/L at the time of 24 h(P < 0.05). Compared with the normal control group,the mRNA and protein expressions of FN increased time-dependently in HPMCs treated with HGPDS(P < 0.05),which peaked at 6 h and 24 h respectively. The fluvastatin significantly inhibited the effects of HGPDS on the expression of FN in a dose-dependent manner(P < 0.05). Conclusion:HGPDS can increase FN expression in HPMCs,which will be inhibited by Flu.