Objective:To illustrate the influence of ＂autoantibodies＂ in a post-partum period of a Graves’ disease animal model.Methods:Female Balb/c mice aged 6 weeks were randomly divided into experimental group and control group,and injected intramuscularly with Ad-TSHR-289(3.3 × 108 pfu/50 μl PBS) and control adenovirus(Ad-β-gal 3.3 × 108 pfu/50 μl PBS) respectively,three times at 3 weeks intervals.When the immunization was over,both groups were mated with male mice after getting a bit of serum from venae angularis for measurement of TRAb titer and T4 level. The mice were sacrificed 1 day after delivery,blood and thyroids were obtained. T4 level was detected by radio immunoassay. Levels of TRAb,TPOAb,TGAb were detected by electrochemiluminescence immunoassay. The thyroids were used for histological examination. Results:TRAb positive rates were 96.7% in experiment group(P < 0.05). The incidence of hyperthyroidism in mice after delivery was 73.3%,which was higher than that after the 3rd immunization (56.7%). TPOAb positive rates were 16.7% in experiment group. The titre of TGAb in experiment group increased siginificantly higher than that in control group(P < 0.05). Conclusion:An animal model of Graves’ disease is successfully prepared by immunization with Ad-TSHR-289. The TRAb maybe involved in the increase of TPOAb and TGAb in post-partum mice with Graves’ disease.