Objective:To explore the clinical value of chorionic villus cell cytogenetic analysis in early spontaneous abortion between natural and assisted reproductive technology(ART) pregnancy. Methods:Total 108 chorionic villus specimens that were obtained from 107 cases of early spontaneous abortion were cultured for karyotype analysis. Chorionic villus was successfully cultured in 106 cases,with 46 cases of natural pregnancy group(N group) and 60 cases of ART group,in which there were 7 cases of intrauterine insemination and 53 cases of in vitro fertilization coupled with intracytoplasmic sperm injection. Results:①The success culture rate were 98.15%(106/108). ②The normal karyotypes were identified in 28 cases of N group and 41 cases of ART group. The abnormal karyotypes were identified in 18 cases of N group and 19 cases of ART group. The abnormal karyotypes detection rate were 34.91%(37/106) in all cases. The type of numerical abnormalities was counting for 33.96%(36/106) and the type of structural abnormalities accounting for 0.94%(1/106). ③The majority type of abnormal karyotypes was numerical abnormalities,counting for 97.3%(36/37),and trisomies for chromosomes were common,counting for 75.68%(28/37). In N group,there was 1 case of triploids,1 case of sexual chromosome number abnomalities,and 16 cases of autosomal abnormality. In ART group,there were 2 cases of triploids,2 cases of tetraploids,1 case of robertsonian translocation,2 cases of sexual chromosome number abnomalities,and 12 cases of autosomal abnormality. ④ Males embryo were found 47.17%(50/106),females embryo were found 52.83%(56/106),there was no significant difference in sexuality ratio;In chromosome abnormalities,males embryo were found 48.65%(18/37),females embryo were found 51.35%(19/37),there was no significant difference in sexuality ratio of chromosome abnormality. ⑤Mean ages of patients were (29.61 ± 3.60)years old in N group and (31.98 ± 4.38)years old in ART group,with significant differences between 2 groups. Conclusion:Chromosome abnormalities are important factors of early spontaneous abortion,and analysis of chorionic villus chromosome provides an important theoretical basis for clinical etiological diagnosis and next pregnancy treatment.