Objective:To investigate the protein and mRNA expression of monocarboxylate transporters 1(MCT1),CD147 and CD44 in non-small cell lung cancer(NSCLC),and significance of their clinical pathology. Methods:Fifty-six specimens of the NSCLC and peritumoral tissues and twenty-one control specimens of pulmonary benign diseases were studied. The mRNA and protein expressions of MCT1,CD147 and CD44 in specimens were analyzed by real-time quantitative reverse transcriptase polymerase chain reaction(real-time quantitative RT-PCR) and immunohistochemistry. Results:①The positive expression rates of MCT1,CD147 and CD44 were significantly higher in NSCLC tissues than those in the peritumoral and control tissues(P < 0.05);② The MCT1 expression in the cell membrane was correlated with both CD147 and CD44 expressions(P < 0.05);③ The expressions of MCT1,CD147 and CD44 in NSCLC were not correlated with age,gender,lesion dimension and TNM stage,but significantly correlated with histological types of tumor and lymph node metastasis (P < 0.05). And the mRNA and protein expressions of MCT1 and CD44 in NSCLC was correlated significantly with tumor differentiation degree (P < 0.05). Conclusion: MCT1,CD147 and CD44 may participate in the occurrence and development of NSCLC,and may be act as the diagnose and prognosis index of NSCLC.