Objective:To study the expression of hypoxia-inducible factor 1(HIF-1) and vascular endothelial growth factor(VEGF) in myocardium and liver after myocardial ischemia reperfusion injury. Methods:Adult male SD rats were subjected to 30 min myocardial ischemia by ligation of the left anterior descending coronary artery and 6 h (I30minR6h) or 24h (I30minR24h) reperfusion. The protein expression of HIF-1α,HIF-1β and VEGF in myocardium and liver were examined by Western blot,and the mRNA levels of HIF-1α and VEGF were determined by real-time PCR. Results:Compared with sham group,both protein and mRNA levels of HIF-1α and VEGF were increased in the ischemic region of myocardium in I30minR6h,but unaffected in I30minR24h. The level of HIF-1β was not different in myocardium among the three groups. But compared with sham group,there were no differences of the protein expression of HIF-1α,HIF-1β or VEGF in liver after myocardial I30minR6h or I30minR24h. Conclusion:The expression of HIF-1α and VEGF in myocardium were affected in myocardial ischemia reperfusion injury whereas not changed in liver. The affection was associated with the time of reperfusion.