Objective:To investigate the expression of Notch1,Jagged1 and NF-κB in gastric carcinoma and their correlation with clinicopathological features and Helicobacter pylori(Hp) infection. Methods:The expression of Notch1,Jagged1 and NF-κB was detected by tissue microarray and immunohistochemistry in 60 cases of gastric carcinoma,60 cases of adjacent gastric carcinoma and 20 cases of normal gastric mucosa tissues and Hp infection was tested by Warthin-Starry silver stain. Results:The positive rates of Notch1 and Jagged1 in gastric carcinoma tissue(40.0%,70.0%) were lower than those in adjacent gastric carcinoma tissue (81.7%,96.7%) and normal gastric tissue(85.0%,100.0%),the differences were significantly(P < 0.05). The positive rate of NF-κB in gastric cancer tissue(66.7%) was higher than those in adjacent gastric carcinoma tissue(36.7%) and normal gastric tissue(0.0%),the differences were significantly(P < 0.05). The infection rates of Hp in gastric carcinoma,adjacent gastric carcinoma and normal gastric mucosa tissues were 76.7%,93.3% and 10.0%,respectively,there were significant differences among them(P < 0.05). The expression of Notch1,Jagged1 and Hp infection were not correlated with the clinicopathological features,while the expression of NF-κB was significantly correlated with tumor differentiation status and TNM stage(P < 0.005). The expression of Notch1 was positively correlated with Jagged1(r = 0.460,P < 0.05) and negatively correlated with NF-κB(r = -0.361,P < 0.05). No correlation was shown between Hp infection and expression of Notch1,Jagged1 or NF-κB in gastric carcinoma tissues(r = -0.032,r = 0.155,r = 0.028,P > 0.05). Conclusion:The expression of Notch1,Jagged1 and NF-κB in gastric carcinoma is abnormal but not associated with Hp infection. The low expression of Notch1 and Jagged1 may induce the high expression of NF-κB,which result in the occurrence of gastric carcinoma.