Objective:To determine the pathogen distribution and resistance pattern of the positive clinical specimens in Jiangsu Shengze Hospital in 2011. Methods:A total of 2 224 samples were collected from this hospital in 2011. Bacteria and fungus were identified by API systems,bacterial drug sensitivity was determined by disk diffusion method,WHONET5.5 software was used for data analysis. Results:The positive rate was 29.1%(648/2 224)of 2 224 culture specimens. The majority of the positive specimens were from nerve internal division(26.7%),respiratory division(23.8%),intensive care unit(17.6%) and so on. The percentage of Gram positive bacteria were19.8%(128/648),Gram negative bacteria 66.5%(431/648) and fungus 13.7%(89/648). The most freguent pathogen was Escherichia coli,accounting for 16.4%,the next was Pseudomonas aeruginosa(16.0%) as well as Klebsiella pneumoniae(13.6%). In view of the resistance,extended spectrum β lactamases(ESBLs) were produced in 67.0% of E. coli and 62.5% of Klebsiella pneumoniae. The resistant rates of ESBLs producing strains were higher than those non-ESBLs-producing strains,Enterobacteria were highly sensitive to carbapenem(94.0%~100.0%). The resistant rates of non-fermentative Gram negative strains were higher than Enterobacteriaceae strains. Resistance rates of methicillin resistant strains were higher than methicillin sensitive strains,and no resistant strains to vancomycin and teicoplanin were reported. The resistance rate of Enterococcus and Streptococcus to penicillin and erythromycin were higher(> 65%),no strain was found to resistant to vancomycin and teicoplanin Conclusion:Antimicrobial resistance in clinical bacterial isolates was a very serious problem in our hospital. Surveillance of bacterial resistance was important and valuable for understanding the change of pathogenic bacteria and drug resistance. The reasonable antibiotics should be applied to prevent the occurrence of resistant strains.