Objective:To explore the gene polymorphisms distribution of methylenetetrahydrofolate reductase (MTHFR) C677T,A1298C and methionine synthase reductase (MTRR) A66G amomg the Han female in Zhenjiang,Jiangsu. Methods:A cross-sectional study was used. A total of 2 885 oral epithelial cells samples from recruited Han women in Zhenjiang were collected. The detections of MTHFR and MTRR gene polymorphisms conducted with Taqman-MGB technology. The distribution of gene polymorphisms of this study was analyzed and compared with partial regions of China,which included Weifang,Zhengzhou,Deyang and Hainan Province. Results:Among the Han women in Zhenjiang,the frequencies of MTHFR 677CC,CT,TT genotypes were 30.4%,47.8% and 21.8%;the frequencies of MTHFR 1298AA,AC,CC genotypes were 69.1%,27.4% and 3.5%;and the frequencies of MTRR 66AA,AG,GG genotypes were 56.9%,37.1% and 6.0%. Compared with partial other regions,the study found that the frequency of MTHFR 677TT genotype of Han women in Zhenjiang was higher than that in Deyang and Hainan,and lower than that in Weifang and Zhengzhou (all P < 0.01). The frequency of MTHFR 1298CC genotype of Han women in Zhenjiang was lower than that in Deyang and Hainan (all P < 0.01). The genotype of MTRR A66G of Han women in Zhenjiang was lower than that in Hainan(P < 0.01),while no difference was found when compared with other 3 regions (all P > 0.05). Conclusion:The gene polymorphisms distributions of MTHFR C677T,A1298C and MTRR A66G among the Han women in Zhenjiang were found to be statistically different from partial other regions of China.