Objective:To investigate the association of the expression level of serum miRNA and the incidence of pancreatic cancer. Methods: Using TaqMan low density array systematically screened the differentially expressed miRNA between the 24 pancreatic cancer patients and the 24 healthy controls. Then those miRNAs that satisfied the criteria were selected for further individual qRT-PCR confirmation. Results: The expression levels of two miRNAs (miR-451 and miR-409-3p) were confirmed to be significantly different in the pancreatic cancer patients and the controls, and P-values were 0.004 1 and 0.005 7,respectively. The area under the ROC curve (AUC) was 0.938 with sensitivity of 95.8% and specificity of 91.7%. Conclusion: The expression level of serum miR-451 and miR-409-3p can successfully distinguish the pancreatic cancer patients from the healthy.