Objective:To establish the referenced immunophenotyping values of T lymphocytes in the healthy Han nationality from Jiangsu province. Methods:Total 460 peripheral blood samples were collected from the healthy Han nationality (aged from 10 to 64 years old),which were detected by FACSCalibur flow cytometry. The data were analyzed by SPSS16.0. Results:The percentages of CD3+ and CD3+CD4+ T lymphocytes,and CD4+/CD8+ ratio in peripheral blood were significantly higher in female than in male. There was no significant difference in the absolute number of CD3+and CD3+CD4+ T lymphocytes,and the absolute number and the percentage of CD3+CD8+ T lymphocytes between female and male groups. There were obvious differences in the above indexes during different ages,and the absolute numbers of CD3+,CD3+CD8+ and CD3+CD4+ T lymphocytes decreased with the age increasing. The referenced immunophenotyping values of T lymphocytes(P5-P95) in the healthy Han nationality from Jiangsu province were as following. The percentages of CD3+,CD3+CD8+ and CD3+CD4+ T lymphocytes were 50% to 79%,15% to 37% and 25% to 48% respectively in peripheral blood. The absolute numbers of CD3+,CD3+CD8+ and CD3+CD4+ T lymphocytes were 902 to 2 665 cells/μl,281 to 1 106 cells/μl and 453 to 1 365 cells/μl respectively. Moreover,the CD4+/CD8+ ratio was 0.77 to 2.70. Conclusion:The referenced immunophenotyping values of T lymphocytes in the healthy Han nationality from Jiangsu province has been established in the study,which provided important reference to researches and treatments relating to HIV/AIDS.