Objective:To construct PLJM1-p53-GFP plasmid of mouse p53 gene and study the role of p53 in the prostate cancer cells PC3. Methods:The open reading frame(ORF) of mouse p53 gene was amplified from mouse 3T3-L1 cells by RT-PCR,and inserted into the PLJM1-NRG1-GFP vector. The recombinant plasmid was transformed into DH5α competent E. coli. The positive clones were screened by PCR and the inserts were confirmed by DNA sequencing. The PLJM1-p53-GFP plasmid was transfected into the prostate cancer cells PC3. QPCR was used to detect the efficiency of the transfection. The invasive activity and proliferation of PC3 cells were measured after transfecting the PLJM1-p53-GFP plasmid. Results:DNA sequencing demonstrated that the recombinant plasmid PLJM1-p53-GFP was constructed successfully. The invasive activity and proliferation of PC3 cells were obviously decreased after transfection of the PLJM1-p53-GFP plasmid. Conclusion:The plasmid of PLJM1-p53-GFP of mouse p53 gene was successfully constructed. It can be used in the functional research for cancer.