Objective:To co-culture the ventral spinal cord explants and major pelvic ganglion explants from rats and investigate the interaction between co-cultured explants. Methods:We had developed a system for long-term co-culturing of ventral spinal cord explants and major pelvic ganglia explants to observe the variance of neuron growth. Results:Explanted tissues of both types survived well in co-culture. Spinal motor neurons(SMNs) emitted numerous outgrowing processes,some of which associated with neurons in explanted major pelvic ganglia. Conclusion:Some contacts of specific motor neuron-major pelvic ganglia neuron were observed apparently,suggesting that a functional interaction may develop between the spinal motor neurons and major pelvic ganglion neurons in vitro.