Objective:To investigate the distribution and resistance of positive pathogens isolated from blood culture in the First Affiliated Hospital of Nanjing Medical University in 2011. Methods:Automatic blood culture instrument of BACTEC 9240 was used for blood samples; bacteria and fungus were identified by API system and automatic detection machine of VITEK2;bacterial drug sensitivity was determined by disk diffusion method;drug sensitivity of fungi was determined by Rosco method;and Whonet 5.4 software was used for data analysis. Results:Among the 4 051 blood culture samples,602 cases showed positive.Gram-negative bacteria accounted for 47.5%,gram-positive bacteria for 45.0%,fungi for 7.0% and anaerobes for 0.5%. Of the Gram-positive bacteria,61.8% were Staphylococcus,18.0% were Enterococcus and 14.3% were Streptococcus. There was no resistance to linezolid,vancomycin and teicoplanin in Staphylococcus,while nine stains were intermediary sensitive to teicoplanin. The sensitive rate of Enterococcus to linezolid was 100.0%,but two stains of E. faecium were resistant to vancomycin or teicoplanin. The Gram-negative bacteria were composed mainly of Escherichia coli,Klbesiella pneumoniae,Acinetobacter bauamnnii and Pseudomonas aeruginosa. The sensitive rates of Enterobacteriaceae to imipenem and meropenem were 86.8% and 85.8%. Acinetobacter bauamnnii had a relatively high resistance to many antimicrobial drugs,which was above 50.0%. Conclusion:There is a variety of pathogens isolated from the blood culture,which show an increasing trend of the resistance to many antimicrobial agents. Strengthening surveillance allows of no delay.