Objective:To analyze the prevalence and genotype distribution of cervical human papillomavirus (HPV) infection among women in Nanjing,China. Methods:Fast nucleic acid hybridization chip was performed to detect cervical HPV DNA and genotype of 7 531 women and the prevalence and genotype distribution were analyzed. Results:The overall prevalence of cervical HPV infection was 33.0% among women in Nanjing(age-standardized prevalence was 37.3%). Among 2 485 HPV-positive women,the overall prevalence of HPV infection was highest in the group aged ≤24 years(43.3%)and there was significant difference between the prevalence of different age-stratified group;the overall prevalence of multiple genotypes HPV infection was highest in the group aged ≤24 years(40.0%)and there was significant difference between the prevalence of different age-stratified group;among the detected 21 HPV genotypes,the most common high-risk HPV genotypes were HPV16(8.6%),HPV58(7.6%),HPV52(5.2%). Conclusion:The prevalence of cervical human papillomavirus among women aged ≤24 years in Nanjing was relatively high,cervical disease prevention program should give priority to this population;HPV 16,HPV 58 and HPV 52 were the most prevalent cervical HPV genotypes among women in Nanjing.