Objective:To evaluate the diagnosis of melanoma of uvea with color doppler ultrasound and differential diagnosis with other intraocular mass. Methods:Sonogram of 33 patients with melanoma of uvea and 56 patients with other intraocular mass were retrospective analyzed. All diagnosis were confirmed by pathology or following up. Results:Thirty-two patients of uveal melanoma included 32 cases of choroidal melanoma and 1 case of ciliary body melanoma. From them,lesions of 28 patients were hemicycle in shape. The other masses were in mushroom shape. All 32 melanomas of uvea appeared isoechoic or hypoechoic,well-defined boundary lesions in ultrasound. In those,18 tumors were of anterior hyper-echogenicity and posterior attenuation which called scooping phenomenon and 15 tumors had choroidal excavation. Vigorous flow in 25 lesions and manipulus flow in the other 6 lesions from short posterior ciliary arteries could be detected with color doppler flow imaging. All patients had retinal detachment in different level. Other intraocular masses included 6 cases of ciliary body tumors,8 cases were uveal metastatic carcinoma,16 cases were choroidal hematoma,8 cases were choroidal hemangioma and 18 cases were retinoblastoma. Conclusion:High frequency color doppler ultrasonography has great value in the diagnosis and differential diagnosis of uveal melanoma.