Objective:To clarify the changes in the filial mice brain proteins after the pregnant mice exposure to Potassium perfluorooctanesulfonate(PFOS). Methods:The 3 days pregnant mice were randomly divided into 2 groups. The mice in the control group were orally administered with the normal saline. The mice in the other group were orally administered with 0.5mg/kg PFOS for 7 days. The filial mice(within 24 hours) born from the control group(marked as A group) and 0.5 mg/kg PFOS-exposed group (marked as B group) were killed and the brain proteins were collected and homogenized respectively. Changes in brain proteins of the filial mice of A group and B group were examined by shotgun analysis. Results:Compared with A group,the categories of brain proteins in B group were significantly changed and some uncharacterized proteins were detected. Conclusion:Orally administration of PFOS to the pregnant mice results in the abnormal changes in the brain proteins of the filial mice.