Objective:To observe the clinical effect of melanocytes transplantation on vitiligo with coating method in 32 patients.Methods:The medium was prepared with recombinant human granulocyte colony-stimulating factor(rhG-CSF) as the major growth factor,mixed with carbomer gel to constitute the system of coating on the treatment of vitiligo with melanocytes transplantation.Therapeutic evaluation was assessed with certain standard. Results:All patients had repigmentation for more than 20%,in which the repigmentation of six cases was more than 90%,11 cases for 70% to 80% and 15 cases for 30% to 50%. The total effective rate was 100%. The 84.4% of the patients showed evenly repigmentation. No obvious mottling was observed. Conclusion:Melanocytes transplantation on vitiligo with coating method is safe and effective.