Objective:Discuss the methods of plasma membrane and its protein complex separation and qualitative. Methods:We evaluated the application of “density gradient ultracentrifugation”and“aqueous two-phase partition”to separate high-quality plasma membrane. Western blot and transmission electron microscope were used in the following research to validate the purity of the separated cell membrane. Results:We applied the blue-native method to process the one-dimensional separation of the protein complex in the cell membrane and 23 kinds of protein complex were separated. We also processed the second-dimensional separation of the 23 kinds of complex and identified 135 proteins, of which 78 were proteins correlated to plasma membrane and 57 were from endomembrane system. Conclusion:Our results show that the combination of different kinds of purification methods can effectively increase the protein separation rate and separation purity. High purity membrane on plasma membrane proteome research has important significance.