Objective:To investigate the influences of human herpesvirus 6 variant A DR7 gene on the proliferation, migration and invasion of human glioma U87 cells. Methods:Lentiviral vectors pLenti6.3-DR7-IRES2-EGFP was constructed and then transfected to human glioma cells U87. A separate U87 cell line stably expressing DR7 was established after blasticidin screening. The cell proliferation was detected by cell counting Kit-8 and the cell cycle was mearsured by flow cytometry. The migration and invasion abilities were detected by wound healing assay and transwell assay, respectively. Results:The pLenti6.3-DR7-6 × His-IRES2-EGFP lentivitral expression vector was constructed and U87-DR7-EGFP cell line stably expressing DR7 gene was established successfully. Compared with U87-NC-EGFP and U87 cells, The cell proliferation of U87-DR7-EGFP cells was significantly increased. Flow cytometry showed that the overexpression of DR7 increased the proportion of U87 cells at S and G2/M phase compared with U87-NC-EGFP and U87 cells. The percentages of S phase were (34.73 ± 1.12)%, (24.89 ± 0.93)%, (25.39 ± 0.96)% (P < 0.001), and G2/M phase were (17.35 ± 1.61)%, (11.36 ± 1.50)%, (13.17 ± 1.95)% (P < 0.05), respectively. The cure rates were significantly increased in the U87-DR7-EGFP cells compared with U87-NC-EGFP and U87 cells: 6 h cure rates were(33.55 ± 2.83)%, (23.50 ± 3.18)%, (22.03 ± 1.47)% and 12 h cure rates were (70.50 ± 5.39)%, (53.60 ± 4.67)%, (55.09 ± 2.83)%, respectively. The average number of invasive cells in each visual field was significantly increased in the U87-DR7-EGFP cells compared with that of the U87-NC-EGFP and U87 cells (543.00 ± 22.94, 387.00 ± 15.63, 412.00 ± 20.30, P < 0.001). Conclusion:Human herpesvirus 6 variant A DR7 gene promote the proliferation, migration and invasion of human glioma cell U87 in vitro, suggesting that it may play a role in the development of glioma