Objective:To investigate the killer immunoglobulin-like receptor (KIR) genotype in patients with chronic myelogenous leukemia(CML). Methods:The sequence specific primer polymerase chain reaction (SSP-PCR) was employed to identify the KIR genes and pseudogenes in 84 CML patients. Results:The most frequent genotypes were KIR frame genes,including 2DL4,3DL2,3DL3 and 3DP1 (100%),followed by 2DL1,2DL3,2DP1 and 3DL1 (all above 80%). The frequencies of 2DL2 and 2DS2 in CML patients were lower than those in the control group,but there were no statistically difference between the two groups except 2DL5B (OR = 0.208,P < 0.05). Conclusion: KIR 2DL5B may be associatied with CML.