Objective:To analyze the prevalence and integrons distribution of carbapenem-resistant Klebsiella pneumonia (CRKP) which isolated at the affiliated hospital of Nanjing Medical University from 2009 to 2010. Methods:All the isolates were identified by VITEK2 compact or API identification system. The antibiotic susceptibility of 81 CRKP was detected by K-B method. ClassⅠ,Ⅱ and Ⅲ integrons and their variable regions were detected by PCR. Results:The isolating rate of CRKP increased rapidly from 1.6% in 2009 to 11.2% in 2011. Most of the isolates were resistant to the β-lactams. Among the 81 isolates,92.6% of them are detected of intI1 and 78.7% of the isolates are detected of variable regions. The most common gene cassette is aadA2,followed by the aacA4-catB8-aadA1 and aadA2+dfrA25. Conclusion:The proportion of CRKP increased rapidly in our hospital and efficient measures should be carried out to inhibit the dissemination of CRKP;ClassⅠ integrons are common in the CRKP and the isolates have high capability to capture antibiotic resistance gene cassette.