Objective:To observe the expression of microRNA-195(miR-195) in ESCC tissues and the effects of miR-195 on the proliferation of Eca109 cells. Methods:The expression of miR-195 in ten pairs of ESCC tumor tissues and adjacent normal esophageal tissues after surgical resection was detected by Taqman Real-time PCR. The miR-195 mimics was transfected into Eca109 cell by using LipofectamineTM 2000. The ability of cell proliferation and cell cycle were detected by Cell Counting Kit-8(CCK-8)and FACS,respectively. Protein was detected by Western Blot. Results:The miR-195 was down-regulated in ESCC tissues compared with normal esophageal tissues (P < 0.05). After transfected miR-195 mimics,cell proliferation was inhibited by CCK-8 assay and cell cycle was arrested in G0/G1 phase by FACS assay (P < 0.01). Western blot result indicated that Cdc42 protein was reduced after miR-195 mimics transfected (P < 0.01). Conclusion:The miR-195 could induce G1 arrest and inhibit proliferation in Eca109.