Objective:To investigate the effect of IKKα on inflammatory cell infiltration and the expression of inflammatory mediators in the repair phase of ischemia-reperfusion(IR)renal injury in mice. Methods:C57BL/6 mice were divided into 4 groups,including the Sham group,the IR group,the interference group and the control of interference group. The interference group and interference control group were injected with lentivirus in renal two weeks ago. The bilateral renal pedicle was occluded for 20 min and then with reperfusion of 3 days to establish IR renal injury model. The sham group only separated bilateral renal pedicle without clipping. Serum creatinine,blood urea nitrogen and HE staining were preformed to evaluate renal function and morphological changes. The macrophages(F4/80+)infiltration and proliferation factor Ki67 expression were detected by immunohistochemical detection, and the distribution and the expressions of IKKα,p-IKKα and inflammation mediators tumor necrosis factor-α(TNF-α),interleukin-18(IL-18) and IL-1β were detected by Western blot. Results:Compared to the control of interference group and the IR group,the serum creatinine and blood urea nitrogen of the interference group was significantly higher(P < 0.05)and renal pathological damage was severer. The cell proliferation factor Ki67 expression was significantly decreased in the interference group(P < 0.05); the infiltration of macrophage and the expression of inflammatory mediators of TNF-α,IL-18 and IL-1β were increased(P < 0.05). Conclusion:The expression of IKKα has effect and promote the repair of renal ischemia-reperfusion injury.