Objective:To investigate the expression and significance of PTEN and P53 in non-muscle-invasive bladder cancer. Methods:The expression of PTEN and P53 in 121 cases of non-muscle-invasive bladder cancer tissues were determined by the immunochemical SP methods. We combined the clinical follow-up data to analye the relationship between these two markers with tumor stage, grade and recurrence. Results:The expressions of PTEN and P53 protein were detected in 89.2% and 39.6% of bladder cancer specimens, respectively. The positive expression rate of PTEN was negatively correlated with the pathological grades and clinical stages of bladder cancer. However, P53 was positively correlated with the pathological grades and clinical stages of bladder cancer. P53 was significantly correlation with the postoperative recurrence. No significant correlation between PTEN in non-muscle-invasive bladder cancer. Conclusion:The detection of the expression of PTEN and P53 in non-muscle-invasive bladder cancer is helpful to estimate the biological behavior and postoperative recurrence.