Objective:To investigate the sensitivity and specificity of a new insulin resistance index derived from triglycerides and blood glucose through the Botnia clamp and other simple index in clinical application. Methods:A total of 43 subjects were included in the present study. All the patients underwent Botnia clamp. Lipid profiles and other biochemical markers were measured from the fasting blood sample. Correlation coefficient between insulin resistance index and glucose metabolic rate(M value) were studied. Results:The Pearsons’ linear correlation coefficient between TyG and M value (r = -0.536,P < 0.001),HOMA1-IR(r = 0.464,P < 0.01),HOMA2-IR (r = 0.402,P < 0.01),QUICK index(r = -0.448,P < 0.01) and ISI index(r = -0.464,P < 0.01) were statistically significant. The area under ROC curve are TyG(0.879)> HOMA1-IR(0.762)> HOMA2-IR(0.738). The best value of TyG index for the diagnosis of insulin resistance was 4.76,which showed the highest sensitivity(96.4%) and specificity(66.7%). Conclusion:The TyG index is a better index in evaluating the insulin sensitivity in type 2 diabetes. It is accessible and has higher sensitivity and specificity, suggesting that it could be widely used in epidemiological studies or some large-scale clinical trials.