Objective:To analyze the prevalence and predictors of syphilis and herpes simplex type 2 virus (HSV-2) infections among HIV-infected men who have sex with men(MSM) in Jiangsu province. Methods:MSM were convened with Cluster sampling and investigated with follow-up questionnaire survey. We reviewed the case reports of their questionnaire information in Nanjing and Zhenjiang city from March to June,2012. Blood samples were collected to detect syphilis and HSV-2 infections. Results:Total of 264 HIV-infected MSM enrolled in the interview and blood sampling. The prevalence of syphilis and HSV-2 infections was 13.64% (36/264) and 28.03% (74/264),respectively. Multivariate logistic regression analysis showed that active syphilis infection was associated with having more than 3 sex partners in the latest 6 months and HSV-2 infection. The HSV-2 infection was associated with age > 35 years old,locality census register,recent syphilis infection and accepting condom issue service. Conclusion:The HIV-infected MSM in Jiangsu province had high infection rates of syphilis and HSV-2. It is necessary to further strengthen and implement and explore new intervention and testing methods.