Objective:To construct lentivirus-mediated expression vector of Vasohibin-2 (VASH2) and infect hepatocellular carcinoma cell line HepG2,and study its effect on proliferation. Methods:The full-length VASH2 was obtained from total cellular RNA by reverse transcription and PCR. Two restriction enzyme cutting sites NheⅠand PstⅠwere introduced and linked to pTA2 vector. After restriction digestion of pTA2 vector,the target gene was inserted into the digested expression vector Lv-CMV-EGFP. Then the recombinant was evaluated by restriction digestion and sequencing. The recombinant vector,pVSV-G and delta8.91 were co-transfected into 293T cells to produce packed lentivirus. HepG2 was infected with the recombinant lentivirus and screened by flow cytometry.The expression level of VASH2 mRNA and protein in HepG2 was measured by real-time PCR and Western blot. The proliferation was detected by MTT and cell cycle analysis. Results:The lentivirus stably expressing VASH2 were successfully constructed. And the HepG2 proliferation were increased remarkably after infected with the recombinant lentiviral vector. Conclusion:The recombinant lentiviral vector expression VASH2 was successfully constructed and VASH2 can promote the proliferation of HepG2,which will provide a foundation for further study on functions and mechanisms of VASH2 in hepatocellular carcinoma.