Objective:To investigate inhibitory effect of RNPC1a short hairpin RNA (shRNA) on the expression of RNPC1a in cell line SUM1315 and determine the effect of RNPC1a down-expression on the proliferation,migration and invasion of SUM1315 cells. Methods:The RNPC1a shRNA was transfected into SUM1315 cells with lipofectine. Puromycin was used for selecting stable cell line. Real-time PCR and Western blot were performed to analyze the mRNA and protein expression of RNPC1a in SUM1315 cells. Cell proliferation,migration and invasion were assessed by methyl thiazol tetrazolium(MTT),wound-healing experiment and Matrigel invasion assay. Results:The mRNA and protein expressions of RNPC1a gene in the shRNA-RNPC1a-SUM1315 group were significantly lower than those in the control group,confirmed by RT-PCR and Western blot,respectively(P < 0.05). The proliferation of SUM1315 cells was markedly inhibited by RNPC1a shRNA with the inhibition rate at 26.5% (P < 0.05),while scratch repair time extended. Meantime transwell cell invasion assay showed that the invasive ability of SUM1315 cell was also inhibited in the shRNA-RNPC1a-SUM1315 group(23 ± 7),compared with the SUM1315 group(213 ± 12)(P < 0.01). Conclusion:Down-regulation of RNPC1a by RNPC1a shRNA can inhibit the proliferation,migration and invasion of SUM1315 cells.