Objective:To establish an NIH3T3 cell line to stable express human Trop-2 gene,and analysis the affection of Trop-2 on proliferation,migration and aggressiveness of NIH3T3-Trop-2 cell. Methods:The human Trop-2 gene was cloned into eukaryotic expression system pcDNA3.1 and transfected into NIH3T3 cells. The Trop-2 stable expression cells were selected by G418 and confirmed by RT-PCR. The cell proliferation,migration and aggressiveness were detected by MTS and wound healing assay,MMP-2/MMP-9 was analyzed. Results:The proliferation of NIN3T3-Trop-2 was higher than NIN3T3 cell. The wound healing assay results showed and that Trop-2 improved the cell migration,increased the number of foci generated(P < 0.05) and increased MMP-9 expression(P < 0.05) when compared to the NIH3T3 control group. Conclusion:These show that human Trop-2 is sufficient to improve the cell proliferation and induce the transformation of NIH3T3 cells.