Objective:To investigate the androgenic activities of Microcos paniculata,licorice root,Angelica sinensis and the underlying molecular mechanisms. Methods:The effects of extracts on cell viability were examined by an assay based on 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,inner salt(MTS). The expression of androgen receptor target gene was determined by real-time PCR. Results:Three extracts were screened for effects on viability of androgen-responsive 22RV1 cells. The extract of Microcos paniculata enhanced cell viability and the expression of PSA mRNA. But they were blocked by the androgen receptor(AR) antagonist flutamide and by the extracellular-signal-regulated kinase 1/2 (ERK1/2) inhibitor,U0126. Conclusion:Microcos paniculata has androgenic activities in an AR-dependent manner among the three plant extracts. And ERK1/2 signaling participates in Microcos paniculata-induced androgenic effects.