Objective:To study the effects of aplasia rashomolog memberⅠ(ARHI ) on HOC SKOV3 cell lines. Methods:After transfection of the pIRES2-EGFP-ARHI plasmid to the HOC SKOV3 cells with low expression level of ARHI gene, absorbance A was measured and performed to calculate the inhibitory rate of cell growth using CCK-8 method. To analyze the cell cycle distribution and apoptosis rate by using flow cytometry and to test the change in expression level of LC3-Ⅱ protein by using Western blot approach in the pIRES2-EGFP-ARHI-SKOV3 (the test group), pIRES2-EGFP-SKOV3 (the plasmid control group) and SKOV3 (the negative control group) cell groups. Results:After the cells in the test group were cultured for 24, 48, 72, 96 and 120 h, we found that the inhibitory rates of cell growth were 64.69%, 70.17%, 67.01%, 66.87% and 67.70%, respectively. The inhibitory rates of cell growth in the test group were significantly higher than those in the plasmid control group(P < 0.01). The ratios of S phase cells were 64.18%, 38.43% and 15.15%, and the apoptosis rates were 47.97%, 26.53% and 9.33% respectively after culturing the cells for 48 h in the test, plasmid control and negative control group. The proportions of cells in S phase were 43.29%, 10.37% and 10.89%, and the apoptosis rates were 51.34%, 24.70% and 4.39% respectively after culturing the cells in the test, plasmid control and negative control group for 72 h. The significant difference remained between the test and control groups. The expression level of LC3-Ⅱ in test group increased with significant difference compared with the control group after cultured for 48 h. Conclusion:These findings suggest that ARHI gene inhibits the growth of SKOV3 cells, arrests the SKOV3 cells at S phase and induces apoptosis and autophagy.