Objective:To explore the osteogenic differentiation potential of de－differentiated mesenchymal stem cells (De－MSCs). Methods:Compared with MSCs,the proliferation capacity and osteogenic related－gene expression of De－MSCs were detected using CCK8 and qPCR assay. Meanwhile the osteogenic redifferentiation potential was measured by the ALP activity assay and the bone formation was confirmed by alizarin red staining. Results:De－MSCs could express stem cells markers,proliferation capacity was obviously higher than MSCs in osteogenic medium statistically. The expression levels of osteogenic related genes and ALP activities were remarkblely increased compared with MSCs. Red nodules were observed with alizarin red staining after De－MSCs were cultured in osteogenic medium for 28 days. Conclusion:De－MSCs retain some MSC traits,with a higher osteogenic differentiation efficiency than MSCs. De－MSCs could be an alternative potential seed cells in tissue engineering.