Objective:To investigate the effects of perifosine on the growth of human gastric carcinoma cell line SGC7901. Methods:The inhibitory effect of perifosine on the growth of human gastric cancer cell line SGC7901 was observed at different concentrations(0.125,0.250,0.500,0.750,1.500) -滋mol/L by sulforhodamine B(SRB)assay. Plate colony formation assay was performed to detect the colony formation rate. The expression levels of eIF4E and AEG－1 were determined by Real－time PCR and Western blot. Results:Perifosine inhibited the growth of gastric cancer cell line SGC7901 in dose－ and time－dependent manners. The cell colony formation rate was statistically decreased (P < 0.05) as compared with those in the control. The mRNA and protein levels of eIF4E and AEG－1 were reduced gradually dose－dependently. Conclusion:Perifosine,an AKT inhibitor,inhibits the growth and the colony formation rate of human gastric cancer cell via downregulating the expression of eIF4E and AEG－1.