Objective:To investigate the expressions of CXCR4,hTERT and microvascular density(MVD) in gliomas and discuss their relationships with the prognosis of glioma. Methods:Immunohistochemistry was used to detect the expression of CXCR4 and hTERT in 156 human gliomas,MVD was analyzed by VEGF labeling. Their relationships with survival time of patients were also analyzed. Results:① The positive rates of CXCR4 and hTERT in gliomas were 53.8%(84/156) and 50.6%(79/156), respectively. The positive rates of CXCR4 and hTERT in grade Ⅱ,grade Ⅲ and grade Ⅳ of gliomas were 30.8%(16/52),56.5%(39/69),82.9%(29/35),and 26.9%(14/52),53.6%(37/69),80.0%(28/35) respectively. A statistically significance of their expressions was observed among different grades(P < 0.01). ② The average of MVD in 156 gliomas was 44.17 ± 13.43. In grade Ⅱ,grade Ⅲ and grade Ⅳ of gliomas,MVD was 29.85 ± 4.34,50.12 ± 17.80 and 52.53 ± 18.16, respectively(P < 0.01). ③ The median survival time of patients with gradeⅡ,Ⅲ,Ⅳ of gliomas after operations was 30.2,11.7 and 9.8 months,respectively. The median survival time in cases with positive expression of CXCR4,hTERT or MVD≥44.17 were significantly shorter than that in cases with negative expression or MVD< 44.17(P < 0.01). Conclusion:The expressions of CXCR4,hTERT and MVD may be associated with invasive group and tumor angionenesis,their combined assay is helpful for evaluating prognosis.