Objective:To investigate the efficiency of cell strainer－based vitrification in human ovarian tissue cryopreservation,and to find suitable concentration of cryoprotectants. Methods:Human ovarian biopsy tissues were obtained from ten female patients undergoing oophorectomy,endometriosis surgery, and ovarian cystectomy. The ovarian tissues were vitrificated by cryoprotectants containing ethylene glycol (EG),dimethylsulfoxide (DMSO) and sucrose using cell strainer as a device. The cryoprotectant mixtures were formulated as follows:12%DMSO + 12%EG + 0.5 mol/L sucrose,15%DMSO + 15%EG + 0.5 mol/L sucrose,18%DMSO + 18%EG + 0.5 mol/L sucrose,20%DMSO +20%EG + 0.5 mol/L sucrose. The morphology of the primordial follicles,cellular apoptosis in ovarian tissues,estradiol(E2),progesterone(P) and lactate dehydrogenase (LDH) in culture medium were evaluated. The fresh tissues and the tissues in programmed cryopreservation were used as controls. Results:Compared with the fresh group,the proportion of normal primordial follicle declined a little in the cell strainer－based vitrification group but was higher than the programmed cryopreservation group,of which the proportion increased significantly in the 18% and 15% groups. Furthermore,the proportion of TUNEL－positive primordial follicles in the vitrification group was higher than that of the fresh group but was lower than that of the programmed cryopreservation group,of which the proportion decreased significantly in the 15% and 12% groups. Therefore,15% cryoprotectant mixtures was optimum for the ovarian tissues preservation. After recovery culturing the ovarian tissues from vitrification in 15% cryoprotectant mixtures,the levels of E2 and P were similar to the controls. However,the level of LDH was almost same as the fresh group,both of which were much more superior than the programmed cryopreservation group. Conclusion:The cell strainer－based vitrification is superior than the programmed cryopreservation,it could be a new vitrification method for clinical human ovarian tissue cryopreservation. The mixture containing 15%DMSO + 15%EG + 0.5 mol/L sucrose should be a optimized cryoprotectant.