Objective:To induce liver cirrhosis by combined low－dose oral and intraperitoneally injected thioacetamide method,and further investigate the inhibitory effects of docosahexaenoic acid on liver cirrhosis in rat. Methods:One hundred male SD rats were divided into five groups:control group,oral group,intraperitoneally injected group,combined group and treatment group with DHA. Formation rate of liver cirrhosis was observed. Cirrosis nodule and pathological observation of pseudolobule formation was performed. We detected liver function indexes including alanine aminotransferase (ALT),endotoxin determination,liver homogenates superoxide dismutase (SOD) and malondialdehyde(MDA). Results:The toxicity of the combined group is slightly lighter. The mortality in the oral group was 10% (2/20) and cirrhosis rate was 85% (17/20) with one carcinogenesis. Ten weeks after the intraperitoneally injection,the mortality in the oral group was 25% (5/20) and cirrhosis rate was 75% (15/20). In contrast,the mortality in the combined group was 5% (1/20) and cirrhosis rate was 90% (18/20). ALT level and endotoxin of three groups with TAA treatment were significantly higher than those of the normal control group,while there was no significant difference among the three groups with TAA treatment. Furthermore,ALT level and endotoxin of rats treated with DHA were lower than those of three groups with TAA treatment. MDA level of the DHA treatment group was significantly lower than that of the three TAA induced cirrhosis groups, while SOD were significantly higher. Conclusion:Combined low－dose oral and intraperitoneally injection of TAA method successfully induced cirrhosis. DHA inhibited liver cirrhosis induced by combined low－dose oral and intraperitoneally injected thioacetamide method in rat.