Objective:To investigate the reproductive health status and demands among undergraduate students in Nanjing and to improve the level of reproductive health. Methods:Two hundreds and forty－five undergraduates were surveyed anonymously by random sampling from a university of Nanjing. The contents of questionnaires covered the basic cognition of reproductive health,the demands of reproductive health,sexual attitudes,sexual behavior,sexual hygiene and the contact situation of erotic items. Results:Two hundreds and forty－five valid questionnaires (98.00%) were retrieved from all 250 students. The ratio of boys to girls was approximately 1.3∶l.0. Undergraduate students grasped little knowledge about reproductive health. Most students were in favor of knowing more knowledge of reproductive health from schools but not from families, and often ignored the importance of family sex education. Sexual concepts of these students were exposed,and 40.00% undergraduates accepted the premarital sexual behavior. 24.68% subjects had sexual experience,in which only 43.10% used contraception in the first half year. More than 80% students confessed to contact erotic items before. Conclusion:The undergraduates’ reproductive health knowledge is inadequate, and much more education on reproductive health should be reinforced in college. At the same time,we should also pay more attention to family sex education.