Objective:To investigate the effects of new tumor necrosis factor-α-inducing protein (Tip-α) on the expression of cytokines and its significance. Methods:Tip-α were amplified from genome sequence of Helicobacter pylori (H.pylori) strain 26695. The recombinant plasmid was transformed into E.coli. The expression product was purified and confirmed by Western blot. After treated by different concentrations of Tip-α in different times,the level of cytokines(IL-8,TNF-α,IL-1β) in GES-1 were detected by ELISA. They were also detected after NF-κB was inhibited by pyrrolidine dithioearbamate (PDTC,the special inhibitor of NF-κB). Results:It was confirmed by Western blot analysis that Tip-α antibody combined with purified proteins specifically. When the cells were treated with Tip-α,the levels of cytokines significant increased(P < 0.05). After NF-κB was inhibited by PDTC,the levels of cytokines significant decreased (P < 0.05). Conclusion:Tip-α can promote the expression of cytokines(IL-8,TNF-α,IL-1β),it may play an important role in the phlogogenic effect of HP,this effect may be NF-κB signal pathway dependent.