Objective:To investigate the role of heparanase(HPA) gene silencing by miRNA interference on invasion and migration of SACC-M cells. Methods:Expression vectors of recombinant plasmids expressing artificial HPA miRNA were constructed and transfected to knockdown HPA expression in SACC-M cells. Expressions of HPA mRNA and protein were detected by RT-PCR and Western blot,respectively. Matrigel invasion assay and scratch-wound assay were performed to examine the invasive and migration ability of the SACC-M cells. Results:Transfection of recombinant plasmid of pGPH1/GFP/Neo/HPA-siRNA significantly suppressed the HPA mRNA and protein levels. This knockdown significantly inhibited the invasion and migration of SACC-M cells as compared with controls. Conclusion:HPA may play an important role in invasion and migration in human salivary adenoid cystic carcinoma cells and should be considered as a potential small molecule inhibitor therapeutic targeting strategy for the future.