Objective:To observe the differences between serum chemotactic factors and oxidative stress markers in different periods of diabetic nephropathy(DN) and the changes of then after therapy of α-lipoic acid. To explore the correlation of chemotactic factors and oxidative stress markers in diabetic nephropathy and the effects of α-lipoic acid to them. Methods:One hundred patients with type 2 diabetic mellitus were randomly divided into normal group,early DN group and clinical DN group according to 24 hour urinary albumin(24h-UAL). Changes of FKN,MCP-1,GSH-PX,SOD,MDA,24h-UAL,FPG,2hPG,HbA1c and 24h-UAL before and after treatment of α-lipoic acid in three groups were observed. Thirty healthy person were chosen as control group. Results:①Serum GSH-PX and SOD in diabetic groups were significatly lower and serum chemotactic factors and MDA were higher than control group. Serum chemotactic factors and MDA decreased,while GSH-PX and SOD increased significantly after treatment in all groups, 24h-UAL was lower than before in early DN group and clinical DN group. Levels of FKN,MCP-1 and MDA in clinical DN group were obviously higher while GSH-PX and SOD were lower than those in normal two group and early DN group before and after treatment, (P < 0.05). ②Serum chemotactic factors were positivly correlated with MDA,24h-UAL,FPG,2hPG and HbA1c,and negativly correlatied with GSH-PX and SOD. MDA was positivly correlated with 24h-UAL,FPG,2hPG and HbA1c,while GSH-PX and SOD were opposite. Conclusion:Serum chemotactic factors and markers of oxidative stress significantly correlat with 24h-UAL and serum glucose. Serum chemotactic and urine protein are decreased by antioxidant,which may delay the progression of diabetic nephropathy.