Objective:To observe the effect of the solube egg antigen (SEA) on B cell function and the related signal transduction. Methods:In vitro, mouse spleen CD19+ B cells were stimulated by SEA or LPS for 72 hours. Then the expression of CD80, CD86 and CD40 and cell cycle were analyzed by flow cytometry, and the dilution of CFSE was assayed by flow cytometry. At the same time, the IL-10, IL-6, IFN-γ and TGF-β level in cultured supernatants were detected by ELISA. PD98059 (ERK inhibitor), SP600125 (JNK inhibitor), SB203580 (p38MAPK inhibitor) and PDTC (NF-κB inhibitor) were used to study the signal pathways for the induced secretion of IL-10, IL-6 in B cells. Results:The expression of CD80, CD86 and CD40 was up-regulated in B cells stimulated by SEA or LPS. And the ratio of B cells entered S/G2-phase was increased after stimulated by SEA or LPS. SEA or LPS promote B cell proliferation. The IL-10 and IL-6 secretion increased in mouse spleen B cells culture supernatants stimulated by SEA or LPS (P < 0.01). Furthermore, PD98059, SP600125, SB203580 and PDTC inhibited the cytokine secretion in B cells stimulated by SEA or LPS. Conclusion:SEA could up-regulate and promote co-stimulatory molecules expression on B cells and the B cells proliferation. At the same time, SEA could induce the IL-10 and IL-6 secretion of B cells through NF-κB, ERK, JNK and p38MAPK signal transduction pathway.