Investigation on the expression of miR-518d in the placenta and serum of gestational diabetes mellitus and normal pregnant women
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    Abstract:

    Objective:To investigate the expression level of miR-518d in the placenta and maternal serum of gestational diabetes mellitus and normal pregnant women. Methods:The expression of miR-518d in placenta and serum was measured by Taqman RT-PCR in 20 women with GDM and 20 women with normal term pregnancy. Results:Expression of miR-518d in placenta was significantly up-regulated in GDM group(4.35 ± 0.88)compared to control group(1.04 ± 0.73)(P < 0.01). Expression of miR-518d in serum was also up-regulated in GDM group(2.43 ± 1.07)compared to control group(0.96 ± 0.88)(P < 0.05). We also showed that PPARa was a direct target of miR-518d with a specific binding site at the seed sequence. And expression of miR-518d in the placenta was significantly positive correlated with the expression of serum E2(R2=0.759,r = 0.871). Conclusion:miR-518d is related with the mechanism of GDM and we suggest that upregulation of miR-518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.

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薛 璇,王 琪,石中华,赵 纯. miR-518d在正常妊娠及妊娠期糖尿病患者胎盘及血清中的表达研究[J].南京医科大学学报(自然科学版英文版),2013,(11):1572-1575.

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  • Received:June 07,2013
  • Online: November 25,2013
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