Objective:To investigate the underlying mechanisms whereby MDA-MB-231 human breast cancer cell migration is regulated through Wnt5a signaling pathway. Methods:The migration of MDA-MB-231 human breast cancer cells was detected using wound healing assay,and then the migratory ratio of MDA-MB-231 human breast cancer cells blocking the Rac1 expression was measured using siRNA. Results:The activated Rac1 promoted the migration of MDA-MB-231 human breast cancer cells. Blocking of Wnt5a/Dvl2 signaling pathway was capable of retarding the Rac1 activation. Conclusion:We demonstrated that MDA-MB-231 human breast cancer cell migration is regulated by Wnt5a/Dvl2/Rac1 signaling pathway. These findings could provide evidences for designing novel therapy based on inhibition of breast carcinoma metastasis.