Objective:To study the anticancer effect of nano-As4S4/Mn0.5Zn0.5Fe2O4 particles on human cervical cancer cells through chemotherapy in combination with thermotherapy in alternating magnetic fields. Methods:The nano-As4S4/Mn0.5Zn0.5Fe2O4 particles were prepared by improved technique of chemical co-precipitation and impregnation,and characterized by SEM,EDS and atom fluorescence spectrometer. The heat effect and the release ratio of nano-As4S4/Mn0.5Zn0.5Fe2O4 particles were studied under alternating magnetic fields in vitro. The treatment effect of human cervical cancer cells was detected by MTT assay and FCM. Results:The nano-As4S4/Mn0.5Zn0.5Fe2O4 particles had an average diameter of 20~40 nm with stable release ratio of 13.28% for 48 h,and had very distinct heat effect in alternating magnetic fields and could rise to a steady temperature for tumor hyperthermia(41~46℃). The inhibition ratio of the cells exposed to nano-As4S4/Mn0.5Zn0.5Fe2O4 particles was significantly higher than that exposed to nano-As4S4 and Mn0.5Zn0.5Fe2O4 groups (P < 0.05). The apoptosis rate of cells exposed to nano-As4S4/Mn0.5Zn0.5Fe2O4 particles was much higher than that exposed alone to that exposed to nano-As4S4 and Mn0.5Zn0.5Fe2O4 groups(P < 0.05). Conclusion:The nano-As4S4/Mn0.5Zn0.5Fe2O4 particles can be successfully conducted by improved technique of chemical co-precipitation and impregnation,and the nano-As4S4/Mn0.5Zn0.5Fe2O4 particles combined with magnetic hyperthermia can effectively induce cell apoptosis.