Objective:To investigate the effects of gabapentin(GBP) on free calcium concentration[Ca2+]i of injury DRG in an experimental rat model with neuropathic pain following spinal nerve ligation. Methods:Thirty five male SD rats aged 4~6 weeks were used in this study. Left L5 spinal nerve was ligated between DRG and sciatic nerve and cut distal to the ligature to reproduce neuropathic pain model. On the 15th postoperative day,these animals were decapitated. L5 DRGs were isolated and the neurons in the ganglion were enzymatically dissociated in SNL group and Sham group. Flow cytometer was used to monitored the resting[Ca2+]i and high K+(50 mmol/L)-evoked[Ca2+]i with or without GBP in L5 DRG. Results:GBP attenuated high K+(50 mM)-evoked[Ca2+]i in a dose-dependent manner(P < 0.05 or P < 0.01),but no effect on resting[Ca2+]i. Conclusion:GBP significantly inhibited high K+-evoked[Ca2+]i in injured DRG neurons in neuropathic pain rats,which may be a peripheral way of GBP which involved in the anti-nociception mechanism.